苦参碱诱导K562细胞合成γ珠蛋白

Matrine-induced gamma-globin synthesis in K562 cells

目的体外研究苦参碱对K562细胞γ珠蛋白合成的影响。方法以K562细胞为体外模型,采用联苯胺染色方法确定苦参碱诱导K562细胞血红蛋白表达增加的剂量效应和时间效应,进一步应用Westernblotting技术检测血红蛋白F(α2γ2)水平。结果0.1mg/ml苦参碱作用于K562细胞,在96h联苯胺染色阳性细胞率达到15.67%;Westernblotting检测到血红蛋白F表达增加。结论苦参碱在体外可诱导K562细胞γ珠蛋白合成增加,从而使血红蛋白F增加。苦参碱具有与阳性对照丁酸钠相似的诱导γ珠蛋白合成增加的作用。

Objective To study the effects ofmatrine on gamma-globin synthesis in K562 cells in vitro. Methods Benzidine staining was used to study the dose- and time-dependent effects of matrine on hemoglobin synthesis in K562 cells, and Western blotting was performed to determine the level of hemoglobin F（α2γ2）. Results Benzidine staining showed that K562 cells treated with matrine of 0.1 mg/ml had a positivity rate for benzidine （BZ%） of 15.67% at 96 h after the treatment, and Western blotting indicated increased synthesis of hemoglobin F. Conclusion Matrine can induce gamma-globin synthesis and increase hemoglobin F level in K562 cells, the effect of which resembles that of sodium butyrate.