晚期糖基化终产物受体Gly82Ser多态性与2型糖尿病相关性的研究

Association of Gly82Ser polymorphism of receptor for advanced glycation end products gene in a type 2 diabetic Chinese population

目的探讨晚期糖基化终产物受体(RAGE)基因Gly82Ser多态性在中国汉族人中的分布特点和对中国2型糖尿病易感性的影响。方法应用多聚酶链反应-限制性片段长度多态性方法检测194例2型糖尿病患者和546例非糖尿病对照者的Gly82Ser多态性基因型。结果中国人RAGE基因Gly82Ser多态基因型以GG型、等位基因以G型为主,其频率分布显著高于其他国家和种族。2型糖尿病患者和非糖尿病对照者间,RAGE基因Gly82Ser多态性的基因型(GG、GS、SS)频率或等位基因(G、S)分布皆无显著性差异(P>0.05)。结论RAGE基因Gly82Ser多态性与中国2型糖尿病的发病、发展无关,提示RAGE基因Gly82Ser并非中国2型糖尿病的易感基因。然而,与其他国家、种族相比,RAGE基因Gly82Ser多态性在中国汉族人群、中国2型糖尿病人群呈高分布频率。

Objective To assess the distribution frequency of Gly82Ser polymorphism of receptor for advanced glycation end products （RA GR） gene and investigate its association with type 2 diabetic Chinese patients. Methods The allele fiequencies and genotype distribution of Gly82Ser polymorphism of RAGE gene were compared in a case-control study of 194 type 2 diabetic and 546 non-diabetic subjects. PCR-restriction fi, agment length polymorphism （PCR-RFLP） was used for detection of the genotype variants. Results In general Chinese population and type 2 diabetic Chinese patients, the most fi＇equent genotype and allele of RAGR gene Gly82Ser polymorphism were genotype GG and allele G, whose fi＇equency distribution were significantly higher than those in other countries （P〈0.01）. No significantly difference in the genotype frequencies or allele fi＇equencies of Gly82Ser polymorphism were found between the diabetic patients and non-diabetic subjects （P〉0.05）. Conclnsion Gly82Ser polymorphism of RAGE gene does not demonstrate any association with type 2 diabetes in Chinese patients, but high genotype and allele frequencies of Gly82Ser polymorphism occur in Chinese population and type 2 diabetic Chinese patients.