大肠杆菌表达的重组葡激酶-水蛭素融合蛋白的分离纯化及其二聚体分析

Purification of Recombinant Fusion Protein Staphylokinase-Hirudin Expressed by Escherichia coli and Analysis of its Dimer

采用阴离子交换和凝胶过滤色谱法纯化大肠杆菌高密度培养所表达的重组葡激酶-水蛭素融合蛋白(rSFH),经SDS-PAGE和RP-HPLC分析纯度达到98%以上,每升发酵液得率约0.7g。同时利用疏水色谱、MALDI-TOF对纯化过程中出现的rSFH同源二聚体的分析和表面疏水面积的计算及利用高效排阻色谱(HPSEC)分析NaCl、温度对rSFH的可逆二聚化行为的影响,认为疏水作用在rSFH的可逆二聚化行为中发挥着重要作用。

The recombinant fusion protein staphylokinase-hirudin （rSFH） was purified from the high density ,fermented engineered E. coli by means of ion -exchange chromatography （IEC） and gel filtration （GF）. The purity of rSFH reached to more than 98% determined by RP-HPLC and SDS-PAGE, and the yield was up to 0. 7g per liter of fermentation broth. The analysis of homologous dimmer of rSFH appeared during the purification and calculation of the surface hydrophobic area had been carried out by means of hydrophobic chromatography and MALD-TOF. The influence of sodium chloride and temperature on the behavior of rSFH reversible dimerization was analyzed by high performance sized- exclusive chromatography （HPSEC）. It is concluded that the hydrophobic interaction played an important role in the reversible dimerization of rSFH.