摘要
用无血清StemPro-34 SFM培养基培养2～5日龄小鼠精原干细胞,以小鼠睾丸支持细胞(Sertoli)为饲养层,小鼠胚胎成纤维细胞(STO)饲养层做对照,分别用相差显微镜观察,免疫组化法研究Sertoli饲养层对精原干细胞生物学行为的影响。结果发现精原干细胞在Sertoli及STO两种饲养层上的一周内的生物学行为非常相似,但培养1周后,Sertoli细胞作饲养层的培养体系中保留的精原干细胞要比对照组明显增多,约有30%的精原干细胞能存活下来并能维持存活到60d以上。由此得出结论是Sertoli细胞作饲养层明显促进精原干细胞的更新增殖。
Objective Spermatogonial stem ceils （SSCs） dissociated from 2 - 5 days postpartum mice were cultured on Sertoli ceils feeders, to study the effect of Sertoli ceils feeders on culture of mouse spermatogonial stem cells. Methods Specific markers CD9 of mouse SSCs cultured serum-free StemPro-34 SFM culture medium were identified by immunohistochemical assay. Result During the first week of culture, on Sertoli ceils feeders or STO feeders,the biological behaviors of spermatogonial stem ceils showed no obvious difference. After a week of culture, compared with control , there were more number of spermatogonial stem cells remained when they were cultured for 60 days. These ceils were expressed CD9 positive. In conclusion Sertoli ceils can be used as feeders not only to promote survival but also renewal of spermatogonial stem ceils.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第2期95-98,共4页
China Biotechnology
