DNA芯片技术研究进展及其在分子营养上的应用

Advance and Application of DNA Chip Technology in Molecular Nutrition

DNA芯片技术是近年发展起来的又一新分子生物学研究工具,可使研究者得以自动化、快速、平行地对大量的生物信息加以分析,在基因组水平上研究基因表达。这种技术为从基因组水平研究基因表达水平与生理反应及生理状况的改变之间的关系提供了强有力的手段。通过比较不同营养水平或不同环境条件下的组织细胞基因达到表达谱差异,可以从基因组水平阐明各种营养成分或环境因素对动物机体的基因表达的影响,从而进一步揭示营养生理的机制和环境对动物影响的机理。DNA芯片技术为分子营养的研究开辟了一条崭新的道路,在从DNA芯片的原理、种类、实验设计、统计方法及在分子营养上的应用作一综述。

DNA chip technology is revolutionizing many aspects of biological research, allowing the expression of many thousands of gene transcripts to be monitored simultaneously. This provides powerful tools for the genome-wide correlation of gene transcript levels with physiological responses and alteration in physiological states. Comparative approaches to understanding the genome-wide correlation of gene transcript level with different diet nutrition levels and environmental condition can illustrate the mechanism of effect of nutrition and environment effecting on animal. DNA chip technology provides a powerful tool for molecular nutrition. In this review, we show the advance of DNA chip from principle, classify, design experiment, statistic analysis and its application in molecular nutrition.