转GO基因棉花体内H_2O_2含量的时空表达

Expression of Glucose Oxidase in Transfer GO Cotton

研究转GO基因棉花品系B99267-1、B99261及其受体新陆早7号和非转基因棉花品种中棉所12号内源H2O2时空变化。结果表明,在初花期、花铃期和吐絮期三个棉花生育期中,初花期H2O2含量较低,吐絮期最高;各生育期下部和中部叶片H2O2含量普遍高于顶部叶片。棉花叶片组织中GO活性、GO蛋白含量和内源H2O2含量均成正相关,酶活高的叶片GO蛋白含量高、H2O2含量也高。

The temporal spatial expression of Glucose Oxidase gene in trans GO cotton plants and H2O2 temporal spatial variation in cotton plants were studied. It compared trans GO cotton varieties B99267-1 ,B99261 with recipient control Xinluzao7 and conventional variety Zhongmiansuo 12 on the Glucose Oxidase activity in trans GO cotton plants varied in different stages and parts. At the stage of florescence, boll and harvest ,enzyme activity in the stage of florescence was the lowest and in the stage of harvest was the highest. In all stages, enzyme activity in leaves of the top were usually lower than that of middle and down. GO activity positively correlated with GO protein and H2O2 level.