6-BA,NAA和Vc浓度配比对山葵试管苗增殖体系的影响

The Study on Improving Plantlet Propogation Coefficient of Wasabi Through Optimizing Concentration of 6-BA,NAA and Vc

山葵为十字花科山嵛菜属多年生草本植物,具有强烈的香辛味,是一种高级蔬菜和药用植物。在山葵生产中存在的一个最大问题即健壮种苗的供给,通过山葵的茎尖培养建立组培快繁体系是解决该问题的有效手段。本文通过311-A最优回归设计研究6-BA,NAA和Vc浓度配比对山葵试管苗增殖体系的影响,对11种不同配方培养基中山葵苗的增殖系数进行调查,通过DPS3.01数据统计软件建立回归方程,并对回归方程进行主效因子及其互作效应分析。结果发现:当6-BA(X1)、NAA(X2)、Vc(X3)的浓度分别为1.2504mg/L、0mg/L、1.9668mg/L时,增殖系数可以达到6.8380,进一步优化了山葵试管苗增殖的体系。

Wasabi （Eutrema wasabia （Sieb.） Maxim. ） is a member of cruciferae. Wasabi is a rare worldwide vegetable used both for culinary and medicinal purpose for its strong pungent. However, one of the main problems in planting wasabi is the production of wasabi seedlings, in order to resolve efficiently this problem, a shoot-tip culture system have been established. The study focuses on improving plantlet propogation, optimization regress analyse called 311-A was firstly applied in wasabi multiplication culture. The multiplication coefficients of 11 medium with different level 6-BA and NAA and Vc were investigated. The regress equation was deduced on 6-BA （ X1 ）, NAA （ X2 ） and Vc （ X3 ） as variable factor , and single factor effect and mutual effect were analyzed. The result showed that proliferation coefficient might increase to 6. 8380 while MS medium contained 6-BA 1. 2504mg/L and Vc 1. 9668 mg/L.