摘要
根据Genbank上登录的果胶酶基因序列设计三对不同果胶酶片段的引物,从本实验室已筛选的一株具有降解果胶质功能的细菌(暂编号为:C105)中克隆到了果胶裂解酶(PL)和果胶甲酯酶(PE)基因片段。通过分析,PL基因片段与PE基因片段均与来源Erwiniachrysanthemi的果胶裂解酶和果胶甲酯酶基因的同源性很高,达到90%以上。
Three pairs of primers were designed according to the conservative sequence of the reported Pectinase gene in Genbank. The fragments of pectate lyase （PL） gene and pectinesterase （PE） gene were amplified from a stream of bacteria （C105） which has the ability to degrade pectin, The result of the sequence analysis shows that the fragment of PL gene and PE gene are both above 90% homology with those of Erwinia chrysanthemi.
出处
《生物技术通报》
CAS
CSCD
2006年第C00期381-386,共6页
Biotechnology Bulletin
基金
中国热带农业科学院科技基金项目(RKY0528)
