摘要
利用昆明小鼠13.5dpc的胚胎制备小鼠胚胎成纤维细胞,并以小鼠胚胎成纤维细胞作为饲养层;收集3.5dICR小鼠的囊胚和桑椹胚进行体外培养,筛选纯化ES细胞集落,使其稳定传代后,对其形态学和生物学性状进行初步鉴定,获得阳性细胞集落。
The blastocysts of 3.5d from Kunming mice were collected, cultured on the cell feeder layers that were from the blastocysts of 13.5d from Kunming mice. After stably passaged, the embryonic stem cell colonies were rudimentally identified. They all have typical morphological characteristics of ES cells and positive AKP staining.
出处
《生物技术通报》
CAS
CSCD
2006年第C00期422-426,共5页
Biotechnology Bulletin
基金
青岛市科技将才资助项目
关键词
昆明小鼠
胚胎干细胞
饲养层
分离培养
Kunming mice ES cells Fibroblast feeder layers Isolation and culture
