鲤春病毒糖蛋白(G)基因的分离及同源性比较

The Isolation of Glycoprotein G gene of Spring Viraemia of Carp Virus and the Comparation of Sequence Homologous

通过RT—PCR和巢氏PCR方法从疑似鲤春病毒（SVCV）侵染的镜鲤肝组织中获得了鲤眷病毒糖蛋白（G）基因。通过序列测定与分析，所获得的鲤春病毒糖蛋白（G）基因由606个核苷酸组成。编码一个由202个氨基酸组成的糖蛋白。通过NCBI blast与9个来自不同国家或地区的鲤春病毒糖蛋白（G）基因序列比对分析，发现获得的鲤鱼春季病毒糖蛋白G基因DNA序列与美国分离的AY527273株同源性最高为99．8％，与中国分离的AY842485株同源性次高为98．7％，与英国分离的两个序列SVI538065和SVI538066株的同源性为98．2％，而与其它国家的分离株如SVUI8101、SVI318079、SVI538061、SVI538062、SVI538063的同源性最差，仅为89．4％-90．0％。氨基酸同源性分析结果与DNA同源性分析结果一致，所获得的鲤春病毒糖蛋白G基因氨基酸推导序列与其它9个分离株的氨基酸同源性在89．6％-99．5％之间。对SVCV不同分离株遗传变异和进化关系的分析为下一步开展SVCV快速诊断方法的研究和疫苗的研制奠定了基础。

A combination of RT-PCR and nested PCR was used to clone glycoprotein G gene of Spring Viraemia of Carp Virus （SVCV） in infected mirror carp liver tissue. By sequence analyses to know the gene is comprised of 606 nucleotides and encodes a glycoprotein with 202 amino acid composition. Blasted this obtained sequence on NCBI with 9 sequences coming from different country and district and found that the sequence of pMSVCV has a very high nucleotide sequence identity （99.8% -98.2% ）with the glycoprotein isolated in USA,China and United Kingdom. and showed a little lower nucleotide sequence identity （ 89, 4%- 90, 0% ） with other isolations of Finland,Germany,Yugoslavia,Ukraine and Moldova. The analysis result of amino acid identity of these SVCV isolates is concord with nucleotide identity and the amino acid identity of pMSVCV with other 9 isolates is 89.6% - 99.5%. Knowledge of genetic differences among SVCV isolations will be useful in the development of diagnostic methods and elaboration of vaccination programs and prevent and quarantine operating.