聚合酶链反应检测尖锐湿疣组织中HPVDNA

DETECTION OF HPV DNA IN CONDYLOMA ACUMINATA TISSUES BY MEANS OF POLYMERASE CHAIN REACTION

目的为分析尖锐湿疣（ＣＡ）组织中人乳头瘤病毒（ＨＰＶ）感染情况。资料和方法采用聚合酶链反应（ＰＣＲ）技术为１３８例ＣＡ组织中ＨＰＶＤＮＡ进行分型研究，并对比观察３９例宫颈等组织中ＨＰＶ感染情况。结果ＣＡ组织中ＨＰＶ感染率为９６．５％，ＨＰＶ６、ＨＰＶ１１、ＨＰＶ１６、ＨＰＶ１８型感染率分别为６７．３％，６９．２％，７．２％，５．８％；复合型感染率为４７％，以ＨＰＶ６、ＨＰＶ１１型复合感染最为常见。宫颈等组织中ＨＰＶ感染率为１２．８％。结论本技术实验条件要求严格，为ＨＰＶ感染的研究提供了一种快速、敏感、特异的检测方法，推广应用将有助于对感染率日益增高的ＨＰＶ的流行病学的研究及防治。

PURPOSES In order to analyze the infections of human papillomavirus (HPV) in condyloma acuminata tissues.MATERIAL AND METHODS By means of polymeras chain reaction,HPV infections in condyloma acuminata tissues of 138 patients were grouped and studied observation was comparatinely given to HPV infections of the cervical tissues,etc.RESULTS The HPV infection rate in condyloma acuminata is 96.5%,and the infection rates of HPV6,HPV11,HPV16 and HPV18 are respectively 67.3%,69.2%,7.2%and 5.8%.The complex infection rate is 47%,of the complex infections,HPV6 and HPV11 are the most common.The HPV infection rate of cervical tissues,etc.12.8%.CONCLUSION This technique has a strict demand of lab conditions,and provides a quick,sensitive and specific method for the study of HPV infection.Its popularization and application will be helpful to the research,prevention and treatment of HPV infections.