毛木耳种质资源的酯酶同工酶分析

Analysis on Esterase Isozyme of Auricularia.polytricha

采用垂直平板聚丙烯酰胺凝胶电泳技术对毛木耳56个菌株酯酶同工酶的酶谱多样性进行了研究。结果表明，56份材料有一定的遗传变异，共检测到迁移率不同的10条谱带，各菌株分别具有1—6条酶带，共有26种酶谱类型。菌株被分成了8大群：第一群包括黄耳zh等34个菌株；第二群包括白背木耳等8个菌株；第三、第四群分别为黑木耳和915两个单独的菌株；第五群包括99等4个菌株；第六群包括43等4个菌株；第七群包括50385和AP067两个菌株；第八群包括小上3和杂交34两个菌株。酯酶同工酶分析技术是鉴别种厦品种以下菌株的有效手段。

Esterase isozyme zymogram polymorphisms of 56 strains in A. polytricha were investigated using vertical slab polyacrylamide gel electrophoresis. The results showed that there existed diversity among coarse muer strains. Seventy - six zymogram bands were observed and there were 26 zymogram types in all tested strains. Each strain had 1 - 6 bands respectively. Differences of strains were expressed in their respective characteristic zymograms in the study. The results of cluster analysis at 67% similarity level showed that all strains tested could be clustered into 8 groups, i.e. the first group including 34 strains of huang-er zh etc. , the second group including 21 strains of white back moer etc. , the third and the forth group only single strain of A. auricla and 915 respectively, the fifth group including 4 strains of 99 etc., the sixth group including 4 strains of 43 etc. , the seventh group including 2 strains of 50385 and APO67, the eighth group including 2 strains of xiao-shang 3 and hybrid 34. The analytical technique of esterase isozyme was an available method for identifying strains in species and under species.