预处理对乳鼠心肌细胞缺氧复氧损伤的影响及其机理探讨

EFECT OF PRECODITIONING ON ANOXIA-REOXYGENATION INJURY OF NEONATAL RAT MYOCYTE AND THE EXPLOITATION OF THE MECHANISM

目的观察预处理（ＰＣ）对乳鼠心肌细胞缺氧复氧（Ａ／Ｒ）损伤的影响，并探讨其机理。方法采用心肌细胞Ａ／Ｒ损伤模型，用短暂缺氧进行预处理。结果预处理能提高Ａ／Ｒ后心肌细胞存活率，减少细胞ＭＤＡ产生及ＬＤＨ和蛋白质的漏出，激活心肌细胞ＰＫＣ活性。ＰＫＣ抑制剂完全阻断了ＰＣ的细胞保护作用。结论离体心肌细胞存在ＰＣ保护现象。

Objective:To investigate the effect of hypoxic preconditioning(HPC) on anoxia-reoxygenation(A-R) injury of neonatal rat cardiomyocyte A-R,HPC was performed by transient hypoxia.Results:HPC inproved viability of cardiomyocyte during A-R( P <0.01),reduced formation of MDA and leakage of LDH and protein in cells.HPC also activated cardiomyocyte PKC.H (an inhibitor of PKC) abolished the above protection of HPC.Conclusion:There exists protective phenomenon of preconditioning in cardiomyocyte of neonatal rat in vitro and the mechanism involves in activation of PKC.