粒细胞集落刺激因子影响白血病细胞增生的研究

The study of mononuclear proliferation of acute leukemia under G-CSF stimulation

目的探讨粒细胞集落刺激因子（G-CSF）对急性白血病（AL）细胞增生的影响。方法急性髓系白血病（AML）患者30例，急性淋巴细胞白血病（ALL）患者20例，正常对照20例。化疗前留取骨髓5ml，制备其单个核细胞（MNC）悬液，并分别加入不同浓度G-CSF培养24h后用流式细胞仪（FCM）检测其DNA倍体的量。同时检测MNC的G-CSF受体（G-CSFR）表达率。结果骨髓MNC培养24h后各浓度的DNA倍体量在AML随着G-CSF浓度的增加有线性上升趋势，在ALL和正常对照呈直线。骨髓MNCG-CSFR表达率AML（68．59±13．99）％；ALL（1．90±0．93）％；正常对照（70．50±10．80）％。结论G-CSF可促进AML细胞增生，对ALL和正常粒细胞增生无促进作用。G-CSFR于恶性及正常粒细胞表达，淋巴细胞不表达。

Objective To study of mononuclear proliferation of AL under the G-CSF stimulation. Methods AML 30 cases, ALL 20 cases, normal control 20 cases were chosen. Aspiration bone marrow 5 ml before chemotherapy to make out MNC liquid to cultivate 24 hours to join different concentration G-CSF inspectively, and to survey their DNA diploid with FCM, Meanwhile to measure the expression of G-CSFR in MNC with FCM. Results The different G-CSF concentration in AML is linearity increasing with DNA diploid, in ALL and normal control is linearity horizon with DNA diploid. The expression rate of G-CSFR： AML is （68.59±13.99）%; ALL is （1.90±0.93）%; normal control is（70.50±10.80）%, respectively. Conclusion G-CSF can promote proliferation of AML cells and can not promote ALL and normal granuloeyte preliferation. G-CSFR express in AML and normal granulocyte and do not express in ALL.