高效液相色谱法测定芍甘胶囊中甘草铵的含量

Determination of Ammonium Glycyrrhizinate in Shaogan Capsules by HPLC

目的：建立芍甘胶囊中甘草铵的含量测定方法。方法：采用HPLC法，色谱柱为YWG C18（150min×4．6min，5μm），流动相为乙腈-0．017mol／L磷酸水溶液-三乙胺（35：65：0．3）；检测波长为250nm，流速为0．8ml／min。结果：甘草铵在72—216mg／L浓度范围内呈良好的线性关系（r=0．9998），平均回收率为100．42％，RSD为1．84％。结论：本法灵敏、准确、重现性好、精密度高、专属性强，可用于芍甘胶囊中甘草铵的含量测定和质量控制。

OBJECTIVE To establish a determination method of ammonium glycyrrhizinate in Shaogan Capsules. METHODS The determination was carried out by HPLC with a YWG C18 （ 150 mm 4.6mm,5 μm） column, acetonitrile -0.017 mol/L phosphate triethylamine （356： 656：0.3 ） as mobile phase, detection wavelength was at 250 nm, flow rate was 0.8 mL/min. RESULTS Ammonium glycyrrhizinate showed a good linear relationship at the range of 72 - 216 mg/L, r = 0.999 8. The average recovery was 100.42% and RSD was 1.84%. CONCLUSION The method is sensitive, accurate, reproducible, specific and can be used for quality control of Shaogan Capsules.