摘要
由EPA/IARC公布的448种化学物质的诱变试验结果中,经30种以上方法测试的化学物质共有60种。通过MCT对这些物质的诱变终点分析,没有发现能对各种方法均产生阳性结果的化学物质。经两两比较,显示这些化学物质的诱变终点也有很大差别。黄曲霉毒素B_1等有较广泛的诱变终点,而放线菌素D等的诱变终点较为特异。用CASE对其中10种致癌剂分子结构作了分析,提示可能与关键片段不同有关。因此建议在选择筛选方法和阳性对照物时,应该慎重,以免遗漏所谓“窄谱”致癌剂。
Distribution of mutagenic endpoints of 60 chemicals that have been tested with over 30 methods was studied by means of computer automated evaluation. No mutagen could induce positive result in all mutagenic assays. A comparison between every two chemicals also indicated that there were distinct difference in mutagenic endpoints. Analysis of molecular structures of carcinogens showed a specificity of thier carcinogenic substructures. The conclution of this investigation is that the different distribution of mutagenic endpoints would be noticed as selecting a battery of screening methods and a positive control for carcinogenic assay.
关键词
化学致癌剂
诱变终点
计算机自动分析
Chemical carcinogen jMutagenic endpoints
Computer automated structure evaluation
