细胞质因子对香菇交配型因子分离比例的影响

Influence of Cytoplasm Factor on Asymmetric Recovery of Nuclear Types from Dikaryons in Lentinula edodes

主要通过双-单杂交技术,探讨造成香菇原生质体单核化后2种交配型单核体分离比例偏离1∶1与细胞质间的遗传关系.结果表明,双-单杂交所形成的由受体细胞质和受体、供体细胞核组成的同质异核体经原生质体单核化裂解后,毫无例外地都是与受体交配型相同的核居多,说明受体细胞质环境对双-单杂交所得异核体裂解后的核的存活和数量起着重要的作用;双-单杂交中,作为供体的2个核中只有亲和力强的那个核优先迁移到受体中,形成新的异核体,即存在先导核,先导核与B因子的亲和特异性有关,特异性越高,先导核迁移速度越快.

This study has determined the genetic relations between the phenomenon of significant asymmetric ratio of the two component nuclear types from dikaryons and cytoplasm factors in Lentinula edodes via di-mon mating. Genetic analysis to fully compatible di-mon mating showed that the monokaryons with rived dikaryon, this showed that a pre-entrance nucleus exists in donor and pre-entrance nucleus mainly dependent on the compatible specificity of B mating-type factor between acceptor and donor nucleus; the higher the specificity, the faster the migration of pre-entrance nucleus.