大豆异黄酮苷元对糖尿病大鼠血小板聚集的抑制作用

Inhibitory effect of soybean isoflavone aglycones on platelet aggregation in diabetic rats

目的:研究大豆异黄酮苷元对血小板聚集性的影响。方法:普通饲料中添加20g/kg大豆异黄酮苷元混合物,饲喂糖尿病大鼠20周,测定其血小板聚集率。利用ODS柱层析和高效液相色谱技术分离大豆异黄酮苷元,体外观察大豆异黄酮苷元单体对人血小板聚集率的影响。结果:长期食用大豆异黄酮苷元的糖尿病大鼠血小板聚集率显著降低。在人体外血小板聚集实验中,大豆异黄酮苷元均抑制ADP、胶原蛋白和肾上腺素诱导的血小板聚集,其中金雀异黄素为最强,其半抑制浓度(IC50)值分别为0.38,0.30,0.23mmol/L。结论:大豆异黄酮苷元具有血小板聚集抑制效应,在大豆抗血小板聚集中起着重要作用。

Objective： To study the influence of soybean isoflavone aglycones on platelet aggregation. Methods ： Diabetes rats were given diets containing 20g/kg of soybean isoflavone aglycones for 20 weeks, and then the platelet aggregation rates were investigated. Soybean isoflavone aglycones were isolated by ODS column chromatography and high-performance liquid chromatography, and investigated for the inhibitory effects on human platelet aggregation in vitro. Results： During the rats fed with soybean isoflavone aglycones, the platelet aggregation rates were decreased noticeably. In human platelet aggregation assay, isoflavone aglycones inhibited platelet aggregation induced by ADP, collagen and epinephrine, while genistein showed the strongest inhibitory activity with the IC50 value of 0.38, 0.30 and 0.23mmool/L, respectively. Conclusion： Soybean isoflavone aglycones have the function of decreasing platelet aggregation in rates.