铜绿假单胞菌质粒介导的AmpCβ-内酰胺酶耐药性检测

The test of plasmid mediated AmpC β-lactamase resistance in Pseudomonas aeruginose

目的 研究铜绿假单胞菌质粒介导的AmpC β-内酰胺酶的耐药性，检测产AmpC酶株的耐药表型，为临床抗生素的合理使用提供实验室依据。方法 收集108株临床分离鉴定的铜绿假单胞菌，采用Kirby-Bauer药敏纸片法和头孢西丁三维试验（cefoxitin three dimensional test）方法检测出产AmpC酶阳性菌株，检测AmpC酶阳性菌株对12种抗生素的耐药表型。结果 在所收集临床分离的108株铜绿假单胞菌中，产AmpC酶铜绿假单胞菌28株，产AmpC酶菌株对多种抗生素耐药。结论 铜绿假单胞菌对多种抗生素耐药，产AmpC酶是铜绿假单胞菌对多种抗生素产生耐药的重要机制。

Objective To study the resistance of plasmid mediated AmpC β-lactamase in Pseudomonas areuginosa and to detect the resistant genotype of productive AmpC strain in Pseudomonas areuginosa,and to supply the laboratory evidence for antibiotic reasonable apply in clinic. Methods 108 strains of clinical isolated Pseudomonas aeruginosa were determined resistance phenotype of antibiotics by Kirby-Bauer disc test, and cefoxitin three dimensional test were applied to filter AmpC positive strains. The resistant genotype of AmpC positive strains to twelve different antibiotic drugs were tested. Results There were 28 strains of producting AmpC enzyme among 108 Pseudomonas aeruginosa atrains. Producting AmpC Pseudomonas areuginosa strains displayed multidrug-resisit to antibiotics. Conclusion Pseudomonas aeruginosa presentes multidrug resistant, AmpC type β-lactamases is an important resistant mechanism to antibiotics in Pseudomonas aeruginosa.