胞膜和胞浆分化抗原联合检测在儿童白血病免疫分型中的意义

Significance of detection of membrane and cytoplasmic antigens by flow cytometry in immunophenotyping of acute leukemia in children

目的 探讨胞膜和胞浆分化抗原联合检测在儿童白血病免疫分型检测中的意义。方法 采用流式细胞术，运用三色和四色荧光标记技术，检测44例儿童初诊急性白血病病例。结果 44例急性白血病病例中，采用胞膜抗体即可确诊急性B淋巴细胞白血病20例，急性T淋巴细胞白血病1例，急性非淋巴细胞自血病10例，其余13例病例进一步进行胞浆抗原染色，最后确诊为双表型白血病病例7例（1例为T／髓双表型，6例为B／髓双表型），6例为伴有髓系标记的急性B淋巴细胞白血病。结论 对于跨系表达的急性白血病病例以及形态学难以区分是淋系还是髓系的急性白血病病例，流式免疫分型的胞浆抗原检测起着关键作用，对临床诊疗意义重大。

Objective To explore the significance of combined detection of membrane and cytoplasmic antigens in immunophenotyping of acute leukemia. Methods With tri-color and tetra-color fluorescence staining technology,44 cases associated with acute leukimea were detected by flow cytometry. Results In 44 cases,20 cases of acute B lymphocytic leukemia,1 case of acute T lymphocytic leukemia and 10 cases of acute non-lymphocytic leukemia were diagnosed by staning technology of membrane antibodies. Other 13 cases of samples were further stained with cytoplasmic antigens. 7 cases of acute biphenotypic leukemia （1 case of T/myeloid, 6 cases of B/myeloid） and 6 cases of My＋ acute B lymphocytic leukemia were finally diagnosed. Conclusion In cases of cross lineage associated antigens in aute leukemia and morphology was difficult in diagnosing between lymphoid or myeloid leukemia, cytoplasmic antigens detection of flow cytometry is critical, and significant in aiding to diagnosis and therapy.