摘要
为探讨不同浓度重组人表皮生长因子(rhEGF)对成人皮片体外培养生长的影响。采用约2mm^2的皮片接种到24孔培养板内加入不同浓度的rhEGF培养4天,测量皮片生长面积。结果表明:rhEGF对皮片生长有明显的调控作用,皮片生长面积与rhEGF浓度呈抛物线型相关,当浓度为5μg/L,时,皮片生长面积达到最大值7.08±2.40mm^2,约为对照组(3.64±1.98mm^2)的2倍。提示rhEGF在促进皮肤快速生长方面有潜在的临床应用价值。
We investigated the effect of the different concentrations of recombinant human epider- mal growth factor (rhEGF) on the expansion of human skin in vitro. Pieces of skin about 2mm^2 in area were placed in 24-cell wells and rhEGF in different concentrations was added. The wells were incubated at 37℃ in 5% CO_2 for 4d. The expanded area of each piece of skin was measured. The results indicated that rhEGF possessed a regulating effect on the expansion of skin pieces, and the expanded area was parabolically related with the concentrations of rhEGF. When the rhEGF concentration reached 5μg/L, the maximal area expansion (7.08±2.40mm^2) was found, which was as twice as the area in control group (3.63±1.98mm^2). It was suggested that rhEGF had a potential therapeutic effect in wound heal- ing.
