电子顺磁共振技术检测烧伤大鼠早期血浆NO变化的研究

Measurement of blood NO contents using ESR method in rats after thermal injury

为探讨烧伤早期(72小时内)血浆NO含量变化,采用雄性Wistar大鼠TBSA35％烫伤模型,分为正常组(A组)、LPS组(脂多糖注射组,L组)、单纯烧伤组(B组)、脂多糖+烧伤组(LB组)。分别于伤后1,3,8,12,24,48,72小时应用Hb-NO自旋捕集技术,在77K低温测试了血浆NO浓度的变化规律,及其所产生的NO量与波谱三重峰信号强度的关系。结果表明:①B组大鼠伤后不同的时间内血浆NO与伤前值比较有降低趋势,但除伤后12小时组与伤前比较有显著意义(P<0.05)外,其它各组均无显著差异(P>0.05)。②LPS组血浆NO的产生,与A组比较有显著差异(P<0.05)。LB组与伤后同时间B组比较有显著或非常显著意义(P<0.05或0.01)。提示大鼠早期烧伤后经LPS攻击后可产生过量的NO,这对于研究烧伤感染、内毒素血症有着重要的现实意义。

The contents of nitric oxide (NO) in the blood were measured by electron spin resonance (ESR) method in Wistar rats with 35% TBSA III°burn. NO is endothelium derived relaxing factor(E- DRF) released by vascular endothelial cells. The results showed that: ① Blood NO contents were not found to be significantly increased (72 hours postburn);② Escherichia coli lipopolysaccharide (LPS, en- dotoxin) could induce excessive NO formation in early burns.