特发性血小板减少性紫癜患者血小板相关抗体和淋巴细胞亚群的临床观察

Detection of platelet-associated antibody and lymphocyte subsets in patients with idiopathic thrombocytopenic purpura

目的检测急性特发性血小板减少性紫癜患者血小板相关抗体(PAIg)和淋巴细胞亚群,探讨其在特发性血小板减少性紫癜(ITP)免疫发病机制中的作用及临床意义。方法分别采用酶联免疫吸附法(ELISA)和流式细胞术(FCM)检测20例ITP患者及20例健康人外周血中PAIg和T淋巴细胞表型。结果ITP患者组PAIg明显高于健康人组(P<0.01);ITP患者组CD3+、CD4+、CD4+/CD8+显著低于健康人组(P<0.01),CD8+显著高于健康人组(P<0.01)。结论血小板相关抗体和T淋巴细胞亚群的变化能较好的反映ITP发病的病理机制。

Objective To explore the clinical significance of platelet - associated antibody（PAIg） and T lymphocytes subsets in patients with idiopathic thrombocytopenic purpura （ITP） . Methods PAIgG and T lymphocyte subsets were detected by enzyme linked immunosorbent assay （ELISA） and flow cytometry （FCM）, Results PAIg in ITP group was higher than that in normal group（ P 〈 0. 01 =. The percentage of CD3^＋ , CD4^＋ and the value of CD4^＋/CD8^＋ was lower in ITP patients. The percentage of CD8^＋ and CD19^＋ was higher. There was significant difference between ITP group and normal group （ P〈 0.01 ）. Conclusion PAIg and the changes of T lymphocyte subset can reflect the pathogenesis of ITP.