慢性阻塞性肺病患者PBMCs中CCR5表达及意义

Expression of CCR5 receptor on peripheral blood mononuclear cells in patients with chronic obstructive pulmonary disease

目的探讨趋化因子受体5(CCR5)在慢性阻塞性肺疾病(COPD)发病中的作用。方法密度梯度离心法分离30例慢性阻塞性肺病患者(COPD组)及20例查体健康者(对照组)外周血单个核细胞(PBMCs),采用RT-PCR法测定PBMCs培养前后CCR5 mRNA的表达水平,免疫荧光标记-流式细胞仪检测CCR5阳性细胞比率;将COPD组上述检测结果与肺功能相关指标及诱导痰细胞检查结果进行相关性分析。结果两组PBM-Cs培养后CCR5 mRNA表达水平及CCR5阳性细胞比率均显著升高(P<0.01),COPD组显著高于对照组(P分别<0.05、<0.01);COPD组CCR5阳性细胞比率与诱导痰中巨噬细胞数(AM)、中性粒细胞数(PMN)呈正相关,与FEV1占预计值的百分比(FEV1%)呈负相关。结论CCR5可引起COPD慢性气道炎症,其机制可能为趋化巨噬细胞、淋巴细胞及中性粒细胞。

[ Objective]To investigate the role of CCR5 in the pathogenesis of chronic obstructive pulmonary disease （COPD）. [Methods] Lung function and cytology of induced sputum were performed on 30 patients with COPD. Peripheral blood mononuclear cells （PBMCs） were isolated with density gradient centrifugation. Expression of CCR5 mRNA and the rate of CCR5 positive cells on PBMC before and after cultured was respectively detected by RT-PCR and immunofluorescence marker flow cytometry,and was relatively analyzed with Lung function and cytology of induced sputum.[Results] ①Expression level of CCR5 mRNA and the rate of CCR5 positive cells in the two groups after cultured were significantly increased than those before cultured（P〈0. 01）. Both indexex before and after cultured in COPD group were significantly higher than those in normal control （P〈0.05 and P〈0. 01）. The rate of CCR5 positive cells in peripheral blood of COPD patients was positively correlated with the number of macrophage and neutrophil in induced sputum, and negatively correlated with FEV1%. [Conclusions] The chemotactic factor receptor5 （CCR5） may draw the macrophage, the lymphocyte and neutrophil to cause the airway inflammation of chronic obstructive pulmonary disease.