加味四妙丸有效部位群HPLC指纹图谱归属分析及谱效关系研究

Jiaweisimiao’s HPLC Fingerprint and Associated Pharmacodynamics

目的:明确加味四妙丸有效部位群中指纹图谱共有峰与组方药材有效部位及药效的相关性,表征加味四妙丸有效部位群药效物质基础。方法:用指纹图谱共有峰相对保留时间的相对偏差△t和紫外光谱夹角余弦特征相似度,考察共有峰与药材有效部位的相关性;将加味四妙丸中各有效部位及各组方药中的有效部位进行正交组合,测定指纹图谱及评价抗炎、镇痛及降尿酸作用,运用数理统计学的方法将药理数据与指纹图谱各色谱峰相对面积相关联,研究谱效相关性。结果:该方有效部位群12个共有峰分别来源于组方药苍术皂苷、黄柏皂苷、黄柏生物碱、土茯苓皂苷部位,与抗炎、镇痛、降尿酸作用均有相关性。结论:研究有效部位群指纹图谱共有峰的成分特征及谱效关系,是表征复方整体成分-配伍-药效相关性的有效途径。

The purpose of this study is to understand the therapeutical basis of Jiaweisimiao Decoction, using the chromatographic pharmacodynamic approach. In the study, effective elements of the Jiaweisimiao Decoction are taken as exampies. The fingerprints of both effective and relevant elements of the herb are collected through the HPLC/DAD analysis. Relative deviations of retention time and similarities of peak UV spectra are employed to evaluate the correlativity. Different combinations of effective elements are also designed for the purpose. The fingerprints of different combinations are tested using the HPLC method, and 12 chromatographic peaks have been obtained. Correlation and regression analysis is made to discern the interrelationship between each peak and the pharmacologic data collected. The results have confirmed the sources of 12 chromatographic peaks and their pharmacologic correlativity. Authors, therefore, conclude that studying the fingerprints of effective elements and their pharmacologic correlativity makes a valid approach for understanding the therapeutical basis of TCM.