立体定向技术注射6-OHDA至内侧前脑束建立帕金森病大鼠模型

PD rat model were established by the injection 6-OHDA into the medial forebrain bundle (MFB) with stereotactic technique

目的建立大鼠帕金森病模型,观测其行为学和多巴胺能神经元的变化。方法利用立体定向技术,注射6-OHDA至大鼠脑的内侧前脑束,于注射后2、4、6、8周观测阿朴吗啡诱导的大鼠旋转行为学变化;采用免疫组化染色检测TH的表达,了解中脑黑质多巴胺能阳性神经元变化。结果所有大鼠分别于第2、4、6、8周的进行阿朴吗啡诱导旋转行为测试,67只大鼠中出现16只大鼠(23.8%)旋转圈数>7r/min,并且>210r/30min,为合格的PD大鼠模型。第8周时,大鼠旋转次数平均为10.48±1.91/分。免疫组化检测,成功模型光镜下分别计数双侧黑质TH阳性神经元,损毁侧黑质TH阳性神经元占正常侧的3.8%,即毁损侧TH阳性神经元减少96.2%。结论利用立体定向技术,选择内侧前脑束为靶点,可建立6-OHDA大鼠PD模型。

Objective To establish Parkinson＇s disease rat model and observe the changes of behaviors,nigra dopaminergic neuron of PD rat model. Methods Build up Parkinson＇s disease model rat by direct injection 6-OHDA into the medial forebrain bundle （MFB） with stereotactic technique. Apomorphine （0. 5mg/kg） injected celiac to evocate circle behavior. The changes of nigra dopaminergic neuron in the model rat were examined with immunohistochemistry. Results 6-OHDA was injected into the medial forebrain bundle brain. A total 66. 7% of the rats rotated over 210 turns per 30 min after 6-OHDA lesions. The immunohistochemical staining showed a significant reduction （in 3.8%） of TH-positive neurons in the 6-OHDA injection side compared with the opposite side. Conclusion PD rat model was successfully established by injection of 6-OHDA with the stereotactic technique.