rhbFGF和rhTNF-α对体外人颞下颌关节滑膜细胞增殖的影响

Effects of rhbFGF and rhTNF-α on the Proliferation of Human Temporomandibular Joint Synovial Fibroblasts in Vitro.

目的:探讨rhbFGF和rhTNF-α单独或联合应用对体外培养的人颞下颌关节滑膜细胞增殖的影响。方法:rhTNF-α(5ng/m l)和rhbFGF(5 ng/mL,10 ng/mL)单独或联合作用人颞下颌关节滑膜细胞,用四甲基偶氮唑盐比色法(MTT)测定细胞增殖并绘制细胞生长曲线。利用流式细胞术(FCM)检测细胞的增殖指数(PI)。结果:MTT法显示各实验组可显著促进细胞增殖,联合使用组效果更明显。FCM检测各实验组PI值均高于对照组。结论:在体外rhbFGF单独或者联合rhTNF-α使用可以显著促进人颞下颌关节滑膜细胞增殖。

Objective： To study effects of rhbFGF and rhTNF - α alone or in combination on synovial fibroblasts （SFs） proliferation. Methods： SFs were cultured in vitro, rhTNF- α（Sng/ml） and rhbFGF （5ng/ml, 10ng/ml） alone or in combination were added into the medium. Cell proliferation was detected by MTT assay and cell growth curves were obtained. Proliferation index （PI） was studied from Flow Cytometry. Results： rhTNF- α and rhbFGF alone or in combination enhanced SFs proliferation. The combination was more effective. Conclusion： rhbFGF used in combination with rhTNF- α stimulates SFs proliferation more effectively in vitro.