类风湿性关节炎大鼠模型的制备

The reproduction of an improved model of rheumatoid arthritis in rats

目的建立简便实用的类风湿性关节炎大鼠模型。方法利用小牛软骨Ⅱ型胶原加福氏完全佐剂充分乳化后作为造模剂,对Wistar大鼠足底、尾根部、背部多点皮内注射,7天后加强注射一次。结果二次注射后2周,大鼠四肢严重肿胀,踝关节直径、脚爪体积增大,血清TNF-α水平明显升高,胫骨远端骨小梁吸收破坏,可累及骨皮质,关节软骨可见坏死脱落、纤维化,关节软骨可见坏死脱落、纤维化,关节腔狭窄,动物造模全部成功。结论Ⅱ型胶原加完全费氏佐剂是一种良好的类风湿性关节炎大鼠模型造模剂。

Objective To reproduce a simple and practicable animal model of rheumatoid arthritis （RA） for evaluating the therapeutic effects of recombinant human interleukin-1 receptor antagonist （rhIL-1Ra）. Methods The animal model of RA was reproduced in Wistar mrs by intmdermie injection of an admixture of bovine type Ⅱcollagen and Freunds complete adjuvant on the foot pad, tail, and back of the mrs and the injections were repeated 7 days later. Results Two weeks after the second injection, all the extremities were markedly swollen. The diameter of ankle joints, the level of TNF-α in serum, and the size of the paws were increased obviously. There was destruction and absorption of the bone trabeculae of distal end of the tibia. There was also necrosis and fibrosis of cartilage of the joints. The articular space was narrowed. Thus RA model was successfully reproduced in rat. Conclusion Bovine type Ⅱcollagen and Freunds complete adjuvant were a favorable agent for the reproduction of RA model in rars.