摘要
目的建立HPLC法同时测定复方替米沙坦胶囊中替米沙坦和吲哒帕胺的含量。方法采用Kromasil—ODS C18色谱柱,以乙腈.水.冰醋酸(45:55:0.1)为流动相,检测波长为242nm。替米沙坦、吲哒帕胺分别在50—150μg·ml^-1,r=0.9999;5~151μg·ml^-1,r=0.9998,浓度范围内线性关系良好。替米沙坦、吲哒帕胺的平均回收率分别为99.79%、99.64%,RSD分别为0.21%、0.81%。结果在选定的色谱条件下,替米沙坦和吲哒帕胺可以完全分离。结论方法准确,简便可靠,可作为复方替米沙坦胶囊的质量控制方法。
Aim To establish an HPLC method for determination of telmisartan and indapamide in compound telmisartan capsules. Methods A Kromasil-ODS C18 column was used with the mobile phase of acetonitrile-wateracetate(45: 55: 0.1) at the detection wavelength of 242nm. The calibration curves of telmisartan and indapamide were linear in the concentration range of 50 - 150μg.ml 1 ( r = 0. 999 9) and 5 - 15μg.ml^-1 ( r = 0.999 8) ,respectively. The average recoveries were 99.79% and 99.64% , respectively. Results Telmisartan and indapamide could be separated. Three batches of samples were determined. Conclusion The method is simple, sensitive and reliable. It can be used as a quantitative method in quality control of compound telmisartan capsules.
出处
《解放军药学学报》
CAS
2007年第1期68-70,共3页
Pharmaceutical Journal of Chinese People's Liberation Army