HPLC法测定复方替米沙坦胶囊中替米沙坦和吲哒帕胺的含量

Determination of Telmisartan and Indapamide in Compound Telmisartan Capsules by HPLC

目的建立HPLC法同时测定复方替米沙坦胶囊中替米沙坦和吲哒帕胺的含量。方法采用Kromasil—ODS C18色谱柱，以乙腈．水．冰醋酸（45：55：0．1）为流动相，检测波长为242nm。替米沙坦、吲哒帕胺分别在50—150μg·ml^-1，r=0．9999；5～151μg·ml^-1，r=0．9998，浓度范围内线性关系良好。替米沙坦、吲哒帕胺的平均回收率分别为99．79％、99．64％，RSD分别为0．21％、0．81％。结果在选定的色谱条件下，替米沙坦和吲哒帕胺可以完全分离。结论方法准确，简便可靠，可作为复方替米沙坦胶囊的质量控制方法。

Aim To establish an HPLC method for determination of telmisartan and indapamide in compound telmisartan capsules. Methods A Kromasil-ODS C18 column was used with the mobile phase of acetonitrile-wateracetate（45： 55： 0.1） at the detection wavelength of 242nm. The calibration curves of telmisartan and indapamide were linear in the concentration range of 50 - 150μg.ml 1 （ r = 0. 999 9） and 5 - 15μg.ml^-1 （ r = 0.999 8） ,respectively. The average recoveries were 99.79% and 99.64% , respectively. Results Telmisartan and indapamide could be separated. Three batches of samples were determined. Conclusion The method is simple, sensitive and reliable. It can be used as a quantitative method in quality control of compound telmisartan capsules.