赤芍水提物药物血清促HSC-T6细胞凋亡作用的实验研究

Apoptosis-promoting effect of serum with extract from Radix Paeoniae Rubra on HSC-T6 cells

目的应用流式细胞法,研究灌服赤芍水提物后的犬血清对乙醛造模后的肝星状细胞的促凋亡作用。方法采用乙醛造模后的肝星状细胞株(HSC-T6)作为体外研究模型;以赤芍水提物给彼格犬1次性灌胃,取给药前的血清、给药后2,3h血清作为实验药物血清。以流式细胞法检测以上采血时间点上2%浓度的血清对乙醛造模后的HSC-T6作用72h后的促凋亡作用。结果2,3h两个时间点的含药血清能明显促进HSC-T6细胞的凋亡,和同样条件下用空白血清培养的HSC-T6细胞相比其凋亡比例(apoptosis,APOP)显著性增加(P<0.05)。在药物血清的作用下两个时间点组的HSC-T6细胞在G0/G1期细胞比例明显上升(P<0.05);而S期细胞显著减少(P<0.05);但G2/M期并没有一致性的变化。结论两个时间点的药物血清对HSC-T6细胞都有显著的促凋亡作用,且能显著增加HSC-T6细胞在G0/G1期的比例及显著降低S期的比例,这可能是其抑制HSC-T6细胞增殖及促凋亡的原因之一。

Objective To study the apoptosis-promoting effect of clog serum of extract from Radix Paeoniae Rubra on hepatic stellate cells （HSC-T6） in the clog model Method The hepatic stallate cells （HHSC-T6） were taken as the model in vitro established by using acetaldehyde. The Radix Paeoniae Rubra extract was given to clog orally once only. The serum samples were taken before giving Radix Paeoniae Rubra extract, 2 and 3 hours after giving Radix Paeoniae Rubra extract respectively. The apoptosis-promoting effects of these serum samples （in 2 % concentration） on HSC-T6 after 72 hours in the model were detected by flow cytometry. Resuit The serum sample promote obviously HSC-T6 apoptosis in 2 hour group and 3 hour group. The apoptosis rate was increased remarkably compare with that of the blank group （P〈0.05） The proportion of HSC-T6 in G0/G1 phase were raised obviously in 2 hour group and 3 hour group （P〈0.05）, while reduced in S phase （P〈0.05）. There were no any changes in G2/M phase. Conclusion The serum samples of Radix Paeoniae Rubra extract all have obvious apoptosis-promoting effects in two time points, raise the proportion of HSC-T6 in Go/G~ phase and reduce in S phase, which may be the mechanism of Radix Paeoniae Rubra extract for inhibiting HSC- T6 proliferation and promoting HSC-T6 apoptosis.