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人输卵管积液对小鼠胚胎基质金属蛋白酶分泌的影响

Effect of human hydrosalpinx fluid on secretion of matrix metalloproteinases in mouse blastocysts
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摘要 目的探讨人输卵管积液对小鼠胚胎体外培养胚胎发育及着床能力的影响机制。方法收集人输卵管积液,小鼠促超排卵,自然受精后适时收集囊胚,并将其随机分配到含有不同浓度的输卵管积液的培养液中,进行体外培养,观察囊胚的发育,计算囊胚孵出率;采用逆转录聚合酶链反应及免疫印迹法测定囊胚和培养液中的基质金属蛋白酶9(MMP-9)和基质金属蛋白酶组织抑制因子1(TIMP-1)的含量。结果输卵管积液组的囊胚孵出率低于无积液组,囊胚及培养液中的MMP-9和TIMP-1低于无积液组,并呈剂量依赖性,MMP-9/TIMP-1随积液浓度增加而降低。结论输卵管积液通过降低胚胎MMP-9/TIMP-1的分泌和影响胚胎早期发育潜能,从而影响胚胎着床的能力。 Objective： To determine how hydrosalpinx fluid affects matrix metalloproteinases. Methods： Human hydrosalpinx fluid was collected from the patients. Mouse blastocysts were collected from the superovulated mice following natural fertilization and cultured in vitro with different conCentrations of hydrosalpinx fluid. The development of blastocysts was observed and the hatching rate calculated. The concentrations of MMP-9 and TIMP-1 in the blastocysts and in the culture medium were measured with RT-PCR and western blot. Results： Hydrosalpinx fluid significantly decreased the hatching rate of blastocysts and the concentrations of MMP-9 and TIMP-1 both in blastocysts and culture medium, in a dose dependent manner. Conclusion： Hydrosalpinx fluid might interfere with the blastocyst implantation by decreasing the MMP-9/TIMP-1 secretion and impairing the early development of blastocysts.
作者 许重洁 靳镭
机构地区 华中科技大学同济医学院附属同济医院生殖医学中心
出处 《生殖医学杂志》 CAS 2007年第1期29-34,共6页 Journal of Reproductive Medicine
关键词 输卵管积液 基质金属蛋白酶 着床 Hydrosalpinx fluid Matrix metalloproteinases Implantation
分类号 R321.1 [医药卫生—人体解剖和组织胚胎学]
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参考文献15

  • 1Ingersoll EP,Pendharkar NC.Characterization and expression of two matrix metalloproteinase genes during sea urchin development[J].Gene Expr Patterns,2005,5(6):727-732.
  • 2Song W,Jackson K,McGuire PG,et al.Degradation of type IV collagen by matrix metalloproteinases is an important step in the epithelial mesenchymal transformation of the endocardial cashions[J].Dev Biol,2000,227(2):606-617.
  • 3Jastrow N,Chardonnens D,Araman M,et al.Effect of hydrosalpinx fluid on secretion of trophoblastic matrix metalloproteinases[J].Fertil Steril,2002,77(3):588-594.
  • 4Carrasca I,CebraI E,Benitez R,et al.Hydrosalpinx fluid affects murine embryonic development in a coculture system with epithelical endometrial cells[J].Fertil Steril,2001,75(5):1004-1008.
  • 5Bany BM,Harvey MB,Schultz GA,et al.Expression of matrix metalloproteinases 2 and 9 in the mouse uterus during implantation and oil induced decidualization[J].Reprod Fertil,2000,120(1):125-134.
  • 6Bischof PA,Messer A,Campana A,et al.Involvement of trophoblast in embryo implantation:regulation by paracrine factors[J].Reprod Immunol,1998,39(1-2):167-177.
  • 7Hampton AL,Butt AR,Riley SC,et al.Tissue inhibitors of metalloproteinases in endometrium of ovariectomized steroid-treated ewes and during the estrous cycle and early pregnancy[J].Biol Reprod,1995,53(2):302-311.
  • 8Lahav-Barata S,Shiloh H,Koifman M,et al.Early embryo-endometrial signaling modlulates the regulation of matrix metalloproteinases-3[J].Fertil Steril,2004,82[Suppl 3]:1029-1035.
  • 9Wang H,Wen Y,Mooney S,et al.Matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase expression in human preimplantation embryos[J].Fertil Steril,2003,80[Suppl 2]:736-742.
  • 10Nuttal PK,Kennedy TG.Epidermal growth factor and basic fibroblast growth factor increase the production of matrix metalloproteinases during in vitro decidualization of rat endometrial stromal cells[J].Endocrinology,2000,141(2):629-636.

二级参考文献11

  • 1Jeziorska M, Nagase H, Salamonsen L A, et al. Immunolocazation of the matrix metalloproteinases gelatinase B and stromelysin 1 in human endometrium throughout the menstrual cycle [J]. J Reprod Fertil, 1996,107(1):43-51.
  • 2Nuttall R K, Kennedy T G. Epidermal growth factor and basic fibroblast growth factor increase the production of matrix metalloproteinases during in vitro deci-dualization of rat endometrial stromal cells [J]. Endocrinology, 2000,141(2):629-636.
  • 3Noyes R W, Hertig A T, Rock J. Dating the endometrial biopsy [J]. Fertil Steril, 1950,1(1):3-7.
  • 4Huppertz B, Kertschanska S, Demir A Y, et al. Immunohistochemistry of matrix metalloproteinases(MMP), their substrates, and their inhibitors(TIMP)during trophoblast invasion in the human placenta [J]. Cell Tissue Res, 1998,291(1):133-148.
  • 5Aplin J D. Expression of integrin α 6 β 4 in human trophoblast and its loss from extravillous cells [J]. Placenta, 1993,14(2):203-215.
  • 6Bischof P, Haenggli L, Campana A. Gelatinase and oncofetal fibronectin expression is dependent on integrin expression on human cytotrophoblasts[J]. Hum Reprod, 1995,10(3):734-742.
  • 7Damsky C H, Fitzgerald M L, Fisher S J. Distribution patterns of extracellular matrix components and adhesion receptors are intricately modulated during first trimester cytotrophoblast differentiation along the invasive pathway, in vivo [J]. J Clin Invest, 1992,89:210-222.
  • 8Schatz F, Krikun G, Runic R,et al. Implications of decidualization-associated protease expression in implantation and menstruation [J]. Semin Reprod Endocrinol, 1999,17(1):3-12.
  • 9Soini Y, Alarakkola E, Autio-Harmainen H. Expression of messenger RNAs for metalloproteinases 2 and 9, type Ⅳ collagen, and laminin in noenoplastic and metoplastic endometrium. Hum Pathol, 1997,28(2):220-226.
  • 10Salamonsen L A, Butt A R, Hammond F R, et al. Production of endometrial matrix metalloproteinases,but not their tissue inhibitors, is modulated by progesterone withdrowal in an in vitro model of menstruation. J Clin Endocrinol Metab, 1997,82(5):1409-1415.

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生殖医学杂志
2007年 第1期

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