茜素红共振光散射法检测金属硫蛋白

Determination of metallothioneins by resonance light scattering method using alizarin red

目的：建立一种灵敏、简便的测定金属硫蛋白（MTs）的共振光散射（RLS）新方法。方法：在pH2．5Britton—Robinson缓冲溶液中，在十二烷基苯磺酸钠（SDBS）存在下，茜素红（AR）与MTs作用，导致体系的RLS明显增强。结果：在实验最适条件下，MTs—AR—SDBS体系的共振光散射强度随着MTs的加入在505nm处急剧增强，且增加的强度与MTs加入量成线性关系（r=0．9994）。方法的线性范围为0．06～16．5μg／ml，检出限为19．05ng／ml。结论：方法简便、灵敏、选择性好，检测加标样品中MTs，结果较满意。

Objective：To develop a sensitive and simple resonance light scattering method for determination of metallothioneins （MTs）. Methods： In britton - robinson buffer solution of pH 2. 5 and the presence of sodium dodecylbenzene sulfonate （SDBS） , the intensity of system, sresonance light scattering （RLS） was obviously enhanced because of interaction of MTs with alizarin red （AR）. Results：Under the optimal experimental conditions,the enhancement of resonance light scattering of the MTs - AR - SDBS system at 508 nm was proportional to the concentration of MTs in the range of 0. 06 - 16. 5 μg/ml （ r = 0. 9994 ）. The detection limit was 19. 05 ng/ml. Conclusion ：The proposed assay succeeds in spiked sample determination with the advantages of simplicity and high sensitivity and good selectivity.