HPLC法测保健品中红景天甙

Determination of Hongjingtian-dai in health-care food by HPLC

目的：建立快速测定保健品中红景天甙的液相色谱测定方法。方法：准确称取样品0．1—0．5g，经甲醇超声提取处理，定容，过0．45μm滤膜。滤液10μl进HPLC分析。色谱条件：AgilentC18柱（4．6×250mm，5μm），流动相：甲醇-0．25mol／L磷酸二氢钾水溶液（20：80，pH5．8），流速1．0ml／min，柱温40℃，测定波长220nm。结果：红景天甙不同水平的样品回收率为91．2％～104．2％。RSD均小于5．4％。红景天甙标准溶液浓度0～200mg／L，与峰面积呈良好线性关系，相关系数为0．9994，检出限为：0．10mg／L。结论：方法简便快速、准确可靠，可有效监控保健品的质量，防止不法商贩制造假冒伪劣产品。该体系还可同时测定苯甲酸、山梨酸、糖精钠，减少了实验步骤，节省了时间，适于实际工作需要。

Objective： To develop a method for the determination of Hongjingtian - dai in health - care food by HPLC. Methods：The homogenized samples were extracted with methanol and detected directly by HPLC. The chromatographic conditions included an agilent - C18 column （4. 6 × 250 mm,5 μm） with the mobile phase of methanol - KH2PO4 （ 20：80, pH 5.8） and detected using a DA detector at 220 nm. The column temperature was 40℃. The mobile speed was 1 ml/min. Results： This method had good relativity （ r = 0. 9994 ） and good analytic precision （ RSD 〈 5.4% ）. The samples＇ average rates of recovery were 91.2% - 104. 2%. The detection limit was 0. 10 mg/L. Conclusion：The method is simple, sensitive, accurate and reproducible.