改良荧光菌球法检测黄浦江原水中霍乱弧菌及其耐药特征分析

Application of modified immunofluorescence colonies method to detect Vibrio cholerae in natural river and antibiotic resistance analysis

目的：了解本地区自然水体中霍乱弧菌（VC）的分布，对收集的O139群VC进行选择性抗生素耐药性标记的流行病学分析。方法：2000—2004年霍乱流行季节，使用改良免疫荧光菌球法检测黄浦江原水；使用改良K—B法检测收集到的O139群VC两类菌的耐药性。结果：5年间从1963件水样中共检出57株VC，总阳性率2．90％，除1株稻叶1d外其余皆为O1或O139群VC的非流行株；耐药谱显示2000年和2001年的O139病人株对氨基糖甙类、四环素类和呋喃唑酮存在显著性差异（P〈0．05），而部分O139甲鱼株的耐药谱与病人的相近，提示可能为相同来源。结论：改良免疫荧光菌球法可以同时检测水样中的O1和O139群VC，具有可推广性；2000年和2001年O139病人株可能属于不同的克隆群，甲鱼是潜在的传染源。

Objective：To realize the distribution of Vibrio cholerae （VC） in natural water of local area and collection of strains of O139 selectivity to carry out epidemiological analysis with antibiotic resistance mark. Methods：From 2000 to 2004, we detected raw water from Huangpu fiver with modified immunofluorescence colonies method and used modified K - B method to detect anti-biotic resistance of characterization differences of O139 strains stock. Results：The 57 strains of VC was detected from 1963 river samples for 5 years, total positive rate was 2. 90%, that belonged to O1 Eltor and O139 serogroups non -epidemic strains except 1 Eltor inaba ld. Antibiotic resistance spectrum showed O139 patient -isolated strains from 2000 to 2001 had significant difference between aminoglycosides, tetracyclines and furazolidone （ P 〈 0. 05）. The antibiotic resistant rates of some O139 turtle - isolated strains was similar to the patient - isolated＇s, which showed it had the same source. Conclusion：Modified immunofluorescence colonies method could be used for detection of O1 and/or O139 VC from row water samples together and of great worth. O139 patient - isolated strains from 2000 to 2001 may belong to different clones and turtle is potential contaminant.