激光偏振光扫描仪测量正常人视网膜神经纤维层厚度的初步研究

Quantitative measurement of retinal nerve fiber layer thickness by scanning laser polarimetry in normal eyes

目的评价激光偏振光扫描仪GDxVCC在正常人视网膜神经纤维层(Retinalnervefiberlayer,RNFL)厚度测量的可重复性,初步确定GDxVCC测量RNFL厚度的正常值。方法用激光偏振光扫描仪GDxVCC对67例(123眼)正常人进行RNFL厚度测量,重复三次,取平均值。比较性别、眼别间及同一眼内上下方RNFL厚度的差别,对RNFL厚度的重复测量进行组内相关系数计算。结果67例(123眼)正常人的平均RNFL厚度为:上方(70.30±6.76)μm,下方(67.35±6.77)μm,平均(56.87±4.53)μm;平均TSNIT标准差23.68±4.61;平均双眼对称值0.86±0.11。上方RNFL厚度与下方RNFL厚度值比较,有显著性差异(t=4.952,P=0.000)。男女性别间TSNIT参数比较,无统计学差异。左右眼间TSNIT参数比较,下方RNFL及TSNIT标准差有统计学差异(P=0.005,0.002),上方及平均RNFL无统计学差异(P=0.086,0.529)。所有参数的组内相关系数均>0.5,其中上方、下方及平均RNFL厚度测量的组内相关系数均>0.8。结论用激光偏振光扫描仪GDxVCC测量RNFL厚度的可重复性好,但年龄对GDxVCC测量RNFL厚度的影响仍应进一步研究。

Objective To assess the reproducibility of scanning laser polarimetry GDxVCC in retinal nerve fiber layer （RNFL） thickness measurement in normal eyes, and state the normative database of normal people＇s RNFL thickness parameters measured by GDxVCC. Methods One hundred and twenty-three eyes of 67 normal subjects were underwent RNFL thickness measurement by GDxVCC. The measurements were repeated for 3 times, and the average thicknesses were calculated. The differences of RNFL thickness measurements between sex, right and left eyes were compared. The differences of RNFL thickness measurements between superior part and inferior part of the same eyes were also compared. The intraclass correlation coefficients （ICC） of 3 measurements in every eye were calculated. Results The average RNFL thickness of 123 eyes of 67 normal subjects were： （70. 30±6.76） μm in superior part, （67.35±6. 77） μm in inferior part and （56. 87±4. 53）μm in mean; the average TNSIT standard deviation was 23. 68±4.61 ; the average symmetric value of right and left eyes was 0. 86 ±0. 11. There was significant difference between superior part and inferior part of the same eyes （t = 4. 952, P= 0. 000）. And the inferior RNFL thickness and TNSIT standard deviation between right and left eyes showed significant difference （P =0. 005, 0. 002）, while the superior RNFL thickness and mean RNFL thickness between right and left eye showed no statistical difference （P=0. 086, 0. 529）. And there is no significant difference in RNFL thickness parameters between different sexes. The ICC values of all TSNIT parameters were 〉0. 5, and the ICC values of superior, inferior and mean RNFL thickness were 〉0. 8. Conclusion The reproducibility of GDxVCC in retinal nerve fiber layer （RNFL） thickness measurement in normal eyes was very good. But the effects of age on RNFL thickness measurement by GDxVCC need further research.