原发性胆囊癌中PTEN表达的临床研究

Study on the expression of PTEN in human primary gallbladder carcinoma(HGC)

目的研究抑癌基因-10号染色体缺失的磷酸酶及张力蛋白同源物基因(PTEN)在原发性胆囊癌(HGC)中的表达,以探讨其在HGC发生发展中的作用,寻找胆囊癌诊断及预后的指标。方法应用原位杂交(DNA-RNA)技术与免疫组化S-P法分别检测64例HGC中PTENmRNA、PTEN蛋白的表达。以22例胆囊腺瘤及10例慢性胆囊炎作为对照。结果PTENmRNA及蛋白在HGC组织中的阳性表达率(56.3%,59.4%)均明显低于胆囊腺瘤组织(81.8%,90.8%)和慢性胆囊炎组织(100%,100%;P<0.05)。结论PTEN低表达参与了HGC的发生发展,PTEN与HGC分化、转移有关,其蛋白可作为判断HGC预后的指标。

Objective To study the expression of phosphatase and tensin homology deleted on chromosome ten（PTEN） and analyse its roles in HGC oncogenesis,and to discover good marker for diagnosis and prognosis of HGC. Methods The expression of PTEN mRNA and PTEN protein were examined by in situ hybridization（DNA-RNA） and immunohistochemistry（ SP method）respectively in 64 cases of HGC. Twentytwo cases of gallbladder adenoma and 10 cases of chronic cholecystitis were taken as control, Results The positive expression rates of PTEN mRNA and protein in HGC（56, 3% ,59, 4% ） were significantly lower than those in gallbladder adenoma （81.8 % ,90.8 % ） and chronic cholecystitis （ 100%, 100% ）, P 〈 0. 05, Conclusion PTEN may play important role in HGC oncogenesis. The expression of PTEN is related to the differentiation and metastasis of HGC, and may be amarker for prognosis evaluation.