乳腺导管增生癌变过程中HIF-1α的表达变化及意义

Expression and significance of hypoxia inducible factor-1α during breast carcinogenesis

目的探讨乳腺导管增生癌变过程中缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)的表达变化及其意义。方法应用免疫组织化学方法检测HIF-1α在乳腺正常组织及各组病变中的蛋白表达情况。结果乳腺导管原位癌和浸润性导管癌组织中HIF-1α蛋白阳性表达率明显高于其他组织(P<0.01);浸润性导管癌组织中HIF-1α蛋白阳性表达率明显高于乳腺导管原位癌,且其表达与淋巴结转移、组织学分级、TNM分期密切相关。结论HIF-1α的表达强度在一定程度上与细胞的恶性倾向有关,HIF-1α参与了乳腺导管增生癌变的演进过程。

Objective To investigate the expression level of HIF-1α during breast carcinogenesis and tumor progression and its clinicopathological significance. Methods Expression of HIF-1α in invasive breast carcinomas （ n = 74 ）, adjacent ductal carcinoma in situ（DCIS） （ n = 13 ）, DCIS alone（ n = 5 ）, ductal atypical hyperplastic lesions（ n = 24 ）, usual ductal hyperplastic lesions （n = 35 ）and normal breast epithelia （ n = 46） was analyzed by immunohistochemistry using specific monoclonal antibodies. Results HIF-1α was overexpressed in tumor specimens as compared with normal epithelia and ductal hyperplastic lesions. The expression of HIF-1α in invasive breast carcinomas was significantly higher than that in DCIS. HIF-1α expression level was correlated with TNM stage, lymph node metastasis and histological grade（ P 〈 0.05 ）. Conclusion HIF-1α may play an important role in human breast cancer progression.