脉冲电磁场对骨质疏松的生物效应

Bio-effects of Pulsed Electromagnetic Fields on Osteoporosis

电磁场作为非创伤性疗法在骨病治疗中已得到广泛的关注,故清华大学深圳研究生院以近10年的工作为基础,从细胞水平、动物模型和临床研究3个方面总结了脉冲电磁场应用于防治骨质疏松研究所取得的成果。研究发现,电磁场能够促进DNA的合成和影响成骨细胞的增殖和分化,且其效果存在一定的窗口效应;骨质疏松动物模型研究显示,脉冲电磁场能够改善去势诱导骨质疏松模型骨密度;初步临床实验证实了细胞研究和动物模型研究的作用效果,并且能够有效改善骨质疏松病人的疼痛和改善骨密度水平。因此认为脉冲电磁场将在骨质疏松的防治中发挥重要作用。

This paper reviewed the research progress in the effects of pulsed electromagnetic fields on osteoporosis by our team since 1997. To establish the interaction between osteoporosis and pulsed electromagnetic fields, precise cellular response to the electromagnetic fields in vitro was measured. In a cellular level, the experimental results indicate that DNA synthesis of UMR-106 osteoblast-like cells is increased, the proliferation level is raised using MTT test and differentiation is affected by specific electromagnetic fields stimulation. In the research of the differentiation effects of pulsed electromagnetic fields on osteoblast cell-line （SaOS-2）, the alkaline phosphatase activity is improved by 156% compared with baseline and by 53% with control group by 15Hz, 0. 5-0. 8 mT pulsed electromagnetic fields. The ＂window effects＂ of frequency, magnetic induction intensity and exposure time were observed. To verify the results in the cellular level, animal model was conducted to qualify and optimize the therapeutic regimen. The preventive experiments demonstrate that the corrected bone mineral density is higher than that of OVX group （P〈0. 05）. In the further studies, the serum bone marker and biomechanics properties will be tested. Based on the efficacy of cellular level and animal model, the effects of pulsed electromagnetic fields were investigated, the bone mineral density was improved and the pain level was alleviated significantly after treatment. Consequently, it is concluded that the pulsed electromagnetic fields are beneficial to the osteoporosis treatment and can be applied in the near future .