大气压下低温等离子体灭菌消毒技术的研究

Sterilization by Non-thermal Plasma at an Atmospheric Pressure

传统灭菌方法存在的各种弊端限制了其应用范围,为研究新的灭菌技术,能够在短时间内完成灭菌效果而又不损伤医疗器械,并且要降低对医务人员以及环境的损害,在介绍了大气压下低温等离子体灭菌消毒的优点后,设计了等离子体灭菌用高频高压电源和等离子体发生器,实现了大气压下均匀的介质阻挡放电。研究发现:纯Ar放电等离子的灭菌效果远差于φ(O2)=5%的Ar混合气体放电的灭菌效果,而且等离子体灭菌的效果与细菌的种类有关。基于实验结果以及实验所用等离子发生器的特殊结构,可知等离子体灭菌机理主要是细菌与等离子中所含的活性成分发生作用。

Most of conventional sterilization techniques have some disadvantages which may limit their application extensions, Whereas, sterilization by non-thermal plasma does not have these problems ,and it has its own advantages, which will make its application in the sterilization field very wide. This article introduces the advantages of the non-thermal plasma sterilization. It introduces the high frequency and high voltage source and the non-thermal plasma reactor that have been designed, and the uniformity dielectric barrier discharge （DBD} at an atmospheric pressure is realized. The dielectric barrier discharge uses the x%O2 and Ar mixture gas as working gas, where the x% percent does not exceed 5 %. In the sterilization experiment, the Escherichia coil, staphylococcus and microzyme are choosed. The sterilization experiment result is that the kill rate of Escherichia coil using pure Ar discharge plasma is worse than that using the 5%O2 and Ar mixture gas discharge plasma and that the sterilization effect relates with the microorganism kinds. Because the structure of the non-thermal plasma reactor is coaxial, and the microorganism that is killed does not directly contact with the electric field,but is far away from it and just contact with the afterflow plasma , the possible killing mechanism is that the microorganism reacts with the active particles of the nonthermal plasma directly.