栝蒌薤白半夏汤对急性心肌缺血大鼠血管内皮细胞保护作用的实验研究

An Experimental Study of Gualou Xiebai Banxia Tang on Endothelial Cell Protection in Acute Myocardial Ischemic Rats

目的：探讨栝蒌薤白半夏汤(GXBT)对心肌缺血大鼠血管内皮细胞产生的血清一氧化氮(NO)和血浆内皮素(ET)的影响,以阐明GXBT保护内皮和抗心肌缺血的作用机制。方法：将SD系大鼠50只,随机分为：假手术组、模型组、栝蒌薤白半夏汤低剂量组和高剂量组,阳性对照组为地奥心血康,每组10只大鼠,雌雄各半。通过结扎大鼠冠状动脉左前降支造成心肌缺血模型,造模后1h采血检测NO和ET的水平,及血清心肌酶CK和CK—MB的含量。结果：急性心肌缺血模型组ET、CK和CK—MB明显升高,NO明显下降,与假手术组比较,差异有非常显著性意义(P＜0．01)；栝蒌薤白半夏汤组可以对抗ET的升高,以及CK和CK—MB的升高,提高NO的水平,与模型组比较,差异有显著性或非常显著性意义(P＜0．05,P＜0．01)。结论：栝蒌薤白半夏汤可以调节血管内皮细胞产生NO和ET平衡,防止心肌损伤,这是其保护血管内皮功能和抗心肌缺血的作用机制之一。

Objective:To explore the influence of Gualou Xiebai Banxia Tang(GXBT,瓜蒌薤白半夏汤)on serum nitric oxide(NO)and endoplasmic endothelin(ET)produced in vascular endothelial cells of myocardiac isehemic rats so as to explain the mechanism of GXBT on protecting emlothelium and counteracting myocardial ischemia.Methods:50 SD rats were allocated to sham-operation group(SOG),model group(MG),low dosage GXBT group(LGG),high dosage GXBT group(HGG)and positive control medicine group (PCMG)randomly,each greup 10 rats(half female and half male).The myocardial is- Chemic rat models were established by ligation of left anterior descending branch of corenary artery,1h after establishment of models,blood sampling was used to determine the level of NO and ET and the content of serum creatine kinase(CK)and CK-MK Results:The ET. CK and CK-MB of MG were markedly increase as compared with SOG(P＜0.01);in GXBT groups increasing of ET,CK and CK-MB was counteracted,and the level of NO was increased as compared with MG(P＜0.05,P＜0.01).Conclusion:CXBT can balance the production of NO and ET by vascular endothelial cells,and prevent myocardiac damage This is one of mechanism to protect vascular eodothelial functioc and counteract my- ocardial ischemia.