摘要
本文设计四对特异性引物分别扩增了野生型和突变型钝齿棒杆菌精氨酸生物合成基因簇argCJBDFR,对其中发生突变的基因argJ、argB及argF的变化进行了生物信息学比较,并分析了这些突变可能对精氨酸产量提高的贡献,为采用基因工程手段改良菌种提供有益的信息和指导。
In this paper, four pairs of primers were designed for the arginine biosynthetic argCJBDFR gene dusters amplification from C.crenatum A.S 1.542 and C. crenatum A.S.M2, respectively. The mutation genes argJ, argB and argF from C.crenatum A.S.M2 were compared with those from C.crenatum A.S 1.542 on bioinformatics, and were analyzed for the possible contribution to enhance the arginine production. These results will offer useful information and instruction to improve strains by gene engineering.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第3期219-222,共4页
Food Science
关键词
钝齿棒杆菌
精氨酸
生物信息学
Corynbacteriumcrenatum
arginine（Arg）
bioinformatics
