核微孔膜为基础的乳糖-葡萄糖双功能酶电极的研制

Manufacture of Lactose-glucose Bi-function Enzymic Electrodes Based on Nuclear Pore Film

利用酵母破碎液改性后的核微孔膜作为固定载体,戊二醛为交联剂,通过固定葡萄糖氧化酶(GOD)和β-半乳糖苷酶(GAL)构建出过氧化氢双电极系统。研究了该酶电极的性质,以及不同来源GAL抗干扰性的差异。乳糖电极在0.2%～2%和4%～10%内有良好的线性关系,响应时间40s,半衰期15～20d。葡萄糖电极的线性范围0～1%,分辨率达到0.001%,响应时间20s,半衰期大于60d。该系统可同时测定样品中乳糖和葡萄糖的含量,乳糖电极的葡萄糖干扰用差分法消除。

Hybrid biosensor for the detecting of lactose and glucose simultaneously was developed. This biosensor consisting of two electrodes was based on the nuclear pore films modified by the yeast broken liquid and 1-1202 electrodes. Glucose oxidase （GOD） and β - galactosidase（GAL） were immobilized on the membranes by using the glutaric dialdehyde as crosslinking agent. Having studied the response characteristics of the two electrodes, the biosensor shows good linearity among the ranges of 0.2- 2 g/100ml and 4- 10 g/100ml of lactose and 0- 100mg/100ml glucose respectively. The response time is less than 40s for lactose and less than 20s for glucose. The self（half-life）-life of the galactosidase-electrode is between 15 and 20 days while the glucose oxidase-electrode is more than 60 days. The interference of glucose in lactose detecting can be removed through the subtractive operation between the response datas of the two electrodes.