离子选择性电极浓度直读法快速测定火棘果中的微量钙

Determination of Micro-calcium in Pyracantha fortuneana Fruit by Direct-reading Method of Ca Ion Selective Electrode

目的:为火棘果中钙含量的测定提供一种准确、快速、简便、直观的测定方法,并为火棘果功能性成分的开发提供科学依据。方法:用干法灰化处理样品,用钙离子选择性电极浓度直读法快速测定了火棘果中的钙,实验对离子强度调节剂和掩蔽剂的用量,缓冲剂的用量以及最佳适用pH范围、方法回收率、精密度、检出限及线性范围作了探讨。结果:实验结果表明:方法的线性范围为0.731～4017mg/L,检出限为1.21×10-2mg/L,回收率为92.36%～108.50%,方法的RSD%为1.6%(n=6)。结论:该法用于火棘果中钙的测定快速、简便、成本低廉,结果令人满意。

Purposes： To provide a kind of accurate, fast, simple, and direct reading method for calcium content in Pyracantha fortuneana fruit, for its functional, compositions in scientific study. Methods： To measurethe calcium content in Pyracantha fortuneana fruit by calcium-selective electrode-direct reading concentration method. The experiment studied the TISAB （total ionic strength adjustment buffer）, the optimal scope ofpH and the recovery, precision, and detection limit of this method. Results： The linear range is 0.731 -4017mg/L, the detection limit 1.21 - 10^-2mg/L, the recovery is 92.36%- 108.50%, and the RSD% is 1.6%（n=6）. Conclusions： This method is fast and simple for the determination of the calcium in Pyracanthafortuneana fruit. The cost is low and the result is satisfatory.