黑曲霉产纤维素酶液体发酵条件的研究

STUDY ON THE CULTURE CONDITIONS OF ASPERGILLUS TO PRODUCE CELLULASE

以黑曲霉(Aspergillus niger)菌株为实验材料,采用液体深层发酵方法,研究了其所产纤维素酶各组分的条件.结果表明:纤维素粉3%、硝酸钾3%、聚乙二醇0.1%,初始pH6.5,250mL三角瓶装25 mL液体培养基、接种种龄为1d的种子培养液10%、32℃培养5 d,纤维素酶各组分活力达到最高.经过对液体发酵培养基及发酵条件的优化,黑曲霉达到产酶高峰的时间由6 d缩短为5 d,在最佳培养条件下,黑曲霉所产纤维素酶各组分酶活力分别为:羧甲基纤维素酶活力为24.52 u/mL,滤纸分解酶(FPA)活力为6.89 u/mL,β-葡萄糖苷酶活力为20.63u/mL.

The optimal culture get cellulose were as follows： condition of AspergiUus niger was researched. The optimal culture conditions to cellulose powder 3%, KNO3 3%, PEG 0.1%, initial pH6.5, 25 mL medium in 250 mL flask, inoculating 1 day of seeds 10%, 32 ℃ culture it for 5 days. The activities of cellulose compositions reached the maximal values. In the optimal culture condition , the activities of CMcase, FPA and theβ-glucosidase were 24.52 u/mL, 6.89 u/mL and 20.63 u/mL respectively.