高效液相色谱法测定四黄膏中大黄素、大黄酚的含量

Determination of the Contents of Emodin and Chrysophonol in Sihuang Gao by HPLC

目的建立高效液相色谱法测定四黄膏中大黄素和大黄酚的含量。方法高效液相色谱法。Sphefixorb C18柱，流动相为甲醇-0.1％磷酸溶液（90：10），检测波长254nm，柱温为室温。结果大黄素进样量在1．6～8．01μg，大黄酚进样量在3．2～16.0μg内，线性关系良好，相关系数大黄素r=0．9997，大黄酚r=0．9999，平均回收率大黄素为100．59％（RSD=1．19％），大黄酚为100．28％（RSD=1．33％）。结论本法操作简便，结果准确，重现性好，可用于四黄膏的质量控制。

OBJECTIVE To develop an HPLC method for the assay of emodin and chrysophonol in Sihuang Gao. METHODS It was assayed by HPLC. The column was Sphefixorb C18 ,the mobile phase was methanol -0. 1% phosphoric acid solution （90： 10）. The UV detection wavelength was at 254nm. RESULTS Good linearity of emodin was shown within the rang of 1.6μg- 8.0μg （ r = 0.999 7） and chrysophonol within the rang of 3.2μg - 16.0μg （ r =0.999 9）. The average recovery of emodin was 100.59% （ RSD = 1.19% ） and chrysophonol was 100.28% （RSD = 1.33% ）. CONCLUSION The method is simple, accurate and reproducible. It can be used for quality control of Sihuang Gao.