糖槭叶中皂苷的提取分离条件探讨

Extraction and separation of saponins from the Acer saccharum leaf

目的:研究糖槭叶中皂苷的提取分离条件,为糖槭中皂苷的开发提供科学依据。方法:乙醇浸渍提取法,大孔吸附树脂分离法和薄层色谱法(TLC)。结果:在大孔吸附树脂分离过程中,用10%乙醇作流动相可以分离得到糖、皂苷、香豆素;用30%乙醇作流动相可以分离得到糖、皂苷、香豆素;用50%乙醇作流动相可以分离得到糖、皂苷、香豆素、黄酮苷;用70%乙醇作流动相可以分离得到糖、皂苷;用90%乙醇作流动相可以分离得到皂苷。结论:80%乙醇浸渍法提取,大孔吸附树脂分离皂苷部位方法最佳。

Objective： To study extraction and separation of saponins from the leaf of the Acer saccharum and provide the scientific basis for the development of separation of glucoside from Acer saccharum. Method：We used 80% ethyl alcohol immersion method,macroporous resin separation method and the thin layer chromatography （TLC） method. Result：When we separated saponin from the Acer saccharum leaf with macroporous resin,we obtained the amylase,saponin and coumarin by using 10% ethyl alcohol mobile phase,obtained the amylase, saponin and coumarin by using 30% ethyl alcohol mobile phase,obtained the amylase,saponin,coumarin and favonoid glycoside by using 50% ethyl alcohol mobile phase, obtained the amylase and saponin by using 70% ethyl alcohol mobile phase and obtained the aponin by using 90% ethyl alcohol mobile phase. Conclusion：Extracting efficient components from the Acer saccharum leaf by 80% ethyl alcohol immersion method and separating saponin from the Acer saccharum leaf with macroporous resin are the optimal methods.