FAK和VEGF在直肠癌中的表达及其相互关系的研究

The correlation between expression of focal adhesion kinase and vascular endothelial growth factor in human rectal carcinoma

目的:检测粘着斑激酶(FAK)和血管内皮生长因子(VEGF)在直肠癌中的表达及其与侵袭和转移的关系,探讨二者的相关性。方法:采用免疫组织化学SABC法,观察86例直肠癌及30例非直肠癌组织中FAK和VEGF的表达情况。结果:FAK和VEGF在直肠癌中的阳性率分别为80%和59%。在非直肠癌组织中的阳性表达率分别为10%和13%。FAK和VEGF在侵及浆膜层直肠癌病例中的表达明显高于未侵及浆膜层者,二者之间差异性有统计学意义(P<0.05);有淋巴结转移组与无淋巴结转移组比较差异有统计学意义(P<0.05),FAK与VEGF阳性表达呈正相关(P<0.01)。结论:FAK、VEGF在直肠癌的侵袭和转移中起重要作用,二者在直肠癌中表达升高可以作为预测直肠癌侵袭和转移的指标。

Objective： To detect the expression of focal adhesion kinase （FAK） and vascular endothelial growth factor （VEGF） in human rectal carcinoma, to study their correlation in invasion and metastasis. Metheds： The expression of FAK and VEGF in 86 specimens resected from patients with rectal carcinoma and 30 non-rectal carcinoma specimens, was investigated by immunohistochemical SABC method respectively.Results： the positive rate of FAK, VEGF was 80 % and 59% respectively in rectal carcinoma. However, non-rectal carcinoma was 10% and 13 % respectively. The diference was significant （P〈0.05）. Moreover, the expression of them is higher in the specimen which had been discovered invasion into peritoneal than that in the group without invasion and metastasis.The expression of FAK and VEGF has a close relationship to the depth of invasion, lymph node mestastasis （P〈0.05）. The expression of FAK has a positive correlation with expression of VEGF （P〈0.001）. Conclusion： FAK, VEGF play a key role in the invasion and metastasis of rectal carcinoma and might prognosticate invasion and metastasis.