小麦成熟胚愈伤组织的诱导及植株再生体系的研究

Study on Embryogenic Callus Induction and Plant Regeneration from Mature Embryo of Wheat

以小麦的成熟胚为外植体,通过胚性愈伤组织诱导进行植株再生。研究结果表明,在MS+2,4-D(3.0 mg/L)+VB(1 1.0 mg/L)的培养基中,成熟胚可较高频率的诱导产生愈伤组织,诱导率为72.3%,但愈伤组织质量较差;在低浓度2,4-D和添加了ABA的继代培养基中,愈伤组织质量明显改善,胚性愈伤组织增加。筛选继代的胚性愈伤组织置于分化培养基MS+6-BA(1.0 mg/L)+KT(4.0 mg/L)+NAA(0.3 mg/L)中,分化率达73.18%。该研究建立了一套有效的以成熟胚为外植体的小麦组织培养再生体系。

Embryogenic callus induction and plant regeneration was conducted for the first time from mature embryos of wheat in this study. The result showed that the optimal medium for embryogenic callus induction was MS ＋2,4-D （3.0 mol/L） ＋ VB1 （1.0 mg/L）, and the frequency of induction was 72.3 %. But the quality of embryogenic callus was poor. By reducing consistence of 2A-D and adding ABA in subculture medium, the quality of embryogenic callus was improved. The medium for differentiation was MS ＋ 6-BA （1.0mg/L） ＋ KT （4.0 rag/L） ＋ NAA （0.3 mg/L）, and the differentiation frequency of callus was 73.18 %. Medium for regenerated planflet rooting was 1/2 MS medium free of hormone. An effective system of plant regeneration of wheat was established.