mRNA差异显示技术的研究进展及其在植物研究中的应用

Research Advances and Application of DDRT-PCR in Plant Research

综述了mRNA差异显示技术的原理、技术路线、优缺点以及差异显示技术的改进过程,并对其在植物发育、植物抗性机理、植物杂种优势机理以及植物激素诱导的基因表达等植物研究领域中的应用作了简要的介绍。

mRNA differential display （DDRT-PCR） is an effective means to investigate the differential expression of genes. It is broadly used in plant research. In this article the principles, technical line, advantages and disadvantages of the technique as well as the advancement of this technique were briefly reviewed. Application of this technique in plant research was also introduced, such as development of the plant, mechanisms of the plant resistance and the plant heterosis as well as the gene expression induced by hormone.