小鼠黑色素瘤生长动态观察

Dynamic observation of mouse melanoma

目的:观察小鼠黑色素瘤生长特征。方法:常规肌肉接种小鼠黑色素瘤B16细胞,接种后动态观察瘤体生长情况,并解剖观察瘤体结构特征及内部变化情况。结果:接种B16细胞后3d,接种局部出现红肿;瘤体直径达1.5cm左右,肿瘤外层包膜形成;瘤体达3.0cm左右,肿瘤出现内层;解剖发现,大瘤体两层包膜下有囊状结构存在,在瘤体内层包膜下有大量小体积个体细胞团存在。结论:小鼠黑色素瘤体增大主要与包膜及包膜下的细胞生长有关。

Objective To observe the growth characteristics of mouse melanoma. Methods B16 cells were routinely inoculated in mouse muscle, and the growth of melanoma was dynamically observed on different time. Meanwhile, the structure of melanoma and its internal change were examined through operation. Results Three days after incubating B16 cells, the incubating site became red and swelling. When the diameter of tumor became about 1.5 cm, the outer coat of tumor came into being; and the inner coat of tumor formed while the diameter grew to about 3.0 cm. Cysts could be found between the outer and inner coat of large tumor. And some cells with small volume could be seen under the inner coat in a great amount. Conclusion The enlargement of malignant tumors is mainly related with the outer coat and the growth of cells is located beneath the out coat.